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105. 2 Typical choices of sample preparation techniques useful in bioanalysis 9 Dilution followed by injection 9Protein precipitation 9 Filtration 9 Protein removal by equilibrium dialysis or ultrafiltration 9 Liquid-liquid extraction 9 Solid-supported liquid-liquid extraction 9 Solid-phase extraction (off-line) 9 Solid-phase extraction (on-line) 9 Turbulent flow chromatography 9Restricted access media 9Monolithic columns 9Immunoaffinity extraction 9 Combinations of the above Similairity and Dissimilarity A Medicinal Chemists View There are many examples in literature where the introduction of an -OH group into a ligand either causes an increase or a decrease of biological affinities.

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