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Periodic acid-Schiff reagent Incubation of cultured cells with glycoproteins as radioactivebands Treatmentwithappropriate endo- or exoglycosidase or phospholipases Sepharose-lectin column chromatography Compositional analysis following acid hydrolysis Mass spectrometry NMR spectroscopy Methylation (linkage) analysis Amino acid or cDNA sequencing Detects glycoproteins as pink bands after electrophoretic sep- aration.

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